The permanent collection of the Huntsville Museum of Art is comprised of over 3,000 objects, primarily focused on 19th and 20th century American art with an emphasis on art from the Southeast. African, Asian, and European art, from cultures influential on American art, is the Museum’s secondary collecting focus.

The collection holds some 400 works on paper by American artists such as James McNeill Whistler, Reginald Marsh, John Sloan, Robert Rauschenberg, Frank Stella, and Andy Warhol. These works have allowed the Museum to provide its community first-hand access to original works of art by leading 20th century masters.